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Fig. 3 | Annals of Clinical Microbiology and Antimicrobials

Fig. 3

From: CRISPR/Cas12a-based assay for the rapid and high-sensitivity detection of Streptococcus agalactiae colonization in pregnant women with premature rupture of membrane

Fig. 3

Establishing the CRISPR-GBS fluorescence assay. A Determination of the optimal primer sets for CRISPR-GBS fluorescence assay using 100 copies μL−1 DNA template. The fluorescence signals were obtained at 10 min. Dunnett’s multiple comparisons test was used to analyze the difference from NTC. B Optimizing the concentration ratio of crRNA to LbCas12a. Dunnett’s multiple comparisons test was used to analyze the difference from the ratio of 1:4. Error bars in A, B represent the mean ± SD, n = 3 replicates

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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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