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Fig. 1 | Annals of Clinical Microbiology and Antimicrobials

Fig. 1

From: CRISPR/Cas12a-based assay for the rapid and high-sensitivity detection of Streptococcus agalactiae colonization in pregnant women with premature rupture of membrane

Fig. 1

Schematic diagram of the CRISPR-GBS assay. Genomic DNA was extracted and the target gene was amplified by RPA. Positive fluorescence signals were produced when ssDNA probes were collateral cleaved by activated Cas12a after crRNA recognized the PAM sequence of target gene. RPA recombinase polymerase amplification, PAM protospacer-adjacent motif, ssDNA single-stranded DNA

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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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