Prevalence and detection of Tropheryma whipplei in the stools of Korean patients with diarrhea using real-time PCRs

Table 1 RT-PCR targets and their primers for detecting Tropheryma whipplei in this study

Target	Primer sequence (5′–3′)		Product size, bp	Limit of detection, copies/μL	T_m, °C
Target	Forward	Reverse	Product size, bp	Limit of detection, copies/μL	T_m, °C
16S–23S rRNA ITS	CCGGTGACTTAACCTTTTTGGAGA	TCCCGAGGCTTATCGCAGATTG	489	190	84
rpoB	CTCGGTGTTGATGTTGATCCAA	GCACCGCAACCTCGGAGAAA	109	1830	63
hsp65	CGCGAAAGAGGTTGAGACTG	ACATCTTCAGCAATGATAAGAAGTT	357	26	82
Dig15	TGTTTTGTACTGCTTGTAACAGGATCT	GATGATAGGAGGGATAGAGCAGGA	155	599	74

ISSN: 1476-0711