Fig. 4

Hyaluronidase enzyme activity (boxplot: median, thick line; mean, large dot) is shown for each isolate by invasive GBS isolate group (maternal, stillbirth, EOD and LOD groups) in comparison to the GB37 wild-type (high hyaluronidase activity) and GB37ΔhylB (low hyaluronidase activity). The hyaluronidase enzyme activities of GB37 WT and GB37ΔhylB were assayed on each plate as a control. All GBS isolates with a pigment score greater than zero are shown as small dots. Non-pigmented GBS isolates are shown as blue triangles (Granada pigment score = 0). Note that 451 units of hyaluronidase activity/mg is equivalent to 1 mg/ml of hyaluronidase. We considered a hyaluronidase enzyme activity of > 10 mg/mL as “high”