Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis

Long, Li-Jun; Lin, Min; Chen, Yu-Ran; Meng, Xin; Cui, Ting-Ting; Li, Ya-Ping; Guo, Xu-Guang

doi:10.1186/s12941-022-00522-6

Annals of Clinical Microbiology and Antimicrobials

Table 1 The detailed characteristics of the included studies

From: Evaluation of the loop-mediated isothermal amplification assay for Staphylococcus aureus detection: a systematic review and meta-analysis

Author	Year	Study design	Country	Source of specimens	Gold standard	Type of LAMP	Bacterial species	TP	FP	FN	TN
Su	2014	Prospective	China	Clinical	Culture and latex agglutination^a	orfX-LAMP	MRSA	557	0	9	101
Hanaki (a)	2011	Prospective	Japan	Clinical	PCR	LAMP	S. aureus	195	0	0	10
Hanaki (b)	2011	Prospective	Japan	Clinical	PCR	LAMP	MRSA	192	0	0	13
Metwally (a)	2014	Prospective	Egypt	Clinical	Standard procedures^b	LAMP	S. aureus	36	0	0	24
Metwally (b)	2014	Prospective	Egypt	Clinical	Standard procedures^b	LAMP	MRSA	19	0	0	41
Jiang (a)	2020	Prospective	China	Clinical	Culture^c	m-LAMP-LFB	MRSA	8	0	0	88
Jiang (b)	2020	Prospective	China	Clinical	Culture^c	m-LAMP-LFB	S. aureus	17	0	0	79
Kashani (a)	2020	Prospective	Iran	Clinical	Culture^d	m-LAMP	S. aureus	49	0	0	4
Kashani (b)	2020	Prospective	Iran	Clinical	MIC and disk diffusion	m-LAMP	MRSA	37	4	0	12
Henares	2017	Prospective	Spain	Clinical	Culture^d	eazyplex MRSA test system	S. aureus	5	1	1	44
Rödel (a)	2017	Prospective	Germany	Clinical	Routine species identification^d	eazyplex® MRSA	S. aureus	31	2	0	106
Rödel (b)	2017	Prospective	Germany	Clinical	Routine species identification^d	eazyplex® MRSA	MRSA	6	0	0	25
Nawattanapaiboon (a)^e	2016	Prospective	Thailand	Clinical	Culture^f	LAMP-LFD	MRSA	28	0	2	16
Nawattanapaiboon (b)	2016	Prospective	Thailand	Clinical	Culture^f	LAMP-LFD	MRSA	52	0	0	20
Lim	2013	Prospective	Malaysia	Clinical	PCR	Loopamp DNA amplification kit	S. aureus	99	0	0	25
Leikeim (a)	2019	Retrospective	Germany	Clinical	Culture^g	eazyplex® MRSA and eazyplex® MRSA plus test strips	S. aureus	230	5	1	561
Leikeim (b)	2019	Prospective	Germany	Clinical	Culture^g	eazyplex® MRSA and eazyplex® MRSAplus	MRSA	32	2	0	763
Chen (a)	2020	Prospective	China	Clinical	Culture^h	m-LAMP-LFB	S. aureus	28	0	0	35
Chen (b)	2020	Prospective	China	Clinical	Culture^h	m-LAMP-LFB	MRSA	12	0	0	51

^aColony morphology, Gram staining, testing of catalase, hyaluronidase and coagulase, the Vitek 2 automated system and the API-Staph commercial kit
^bStandard microbiological methods along with MecA PCR
^cCulture, serum agglutination test and Gram stain
^dThe article is not described in detail
^eIt has two sets of data because they come from different samples
^fCoagulase and antimicrobial susceptibility test
^gConventional blood culture diagnostics
^hBlood culture, colony morphology, Gram staining, biochemical identification, and methicillin susceptibility testing

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ISSN: 1476-0711

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