Recurrent bacteremia with a hypermucoviscous Escherichia coli isolated from a patient with perihilar cholangiocarcinoma: insights from a comprehensive genome-based analysis

Table 3 Plasmids present in isolate 537–20

Plasmids	Accession	Size [bp]	Closest Relative(s) based on BLASTN	BLASTN (coverage/identity)	Plasmid replicon type
p537-20_1	CP091535	126,021	pPK8261-180 kb (180,318 bp) pWP5-W18-ESBL-11_1 (158,291 bp) pWP4-S18-ESBL-08_1 (158,292 bp)	CP080157.1 (98/99.79%) AP022121.1 (97/99.88%) AP022096.1 (97/99.88%)	0.99952 identity IncFIC(FII)_1, AP001918 IncFIB(AP001918)_1, AP001918
p537-20_2	CP091536	6,647	pRHB38-C04_2 (6,647 bp) p94EC-5 (6,647 bp) RCS33_pII (6,647 bp)	CP057102.1 (100/100%) CP047581.1 (100/100%) LT985237.1 (100/100%)	1.0000 Identity ColRNAI_1, DQ298019
p537-20_3	CP091537	2,182	pRHBSTW-00087_3 (2,181 bp) p5312.29 (86,455 bp) pWP4-S18-ESBL-09_5 (2,188 bp)	CP056869.1 (100/99.95%) KR905385.1 (82/98.55%) AP022103.1 (63/92.63%)	n.a
p537-20_4	CP091538	1,552	p7_2.5 (1,552 bp) pET6.4-ColRNAI (1,552 bp) pSCU-172–9 (1,552 bp)	CP023825.1 (100/100%) CP043221.1 (100/99.94%) CP054362.1 (100/99.92%)	0.99933 identity Col(MG828)_1, NC_008486
p537-20_5	CP091539	1,433	MINF_9A-sc-2280436, plasmid 9 (1,433 bp) Uncultured metamobilome (1,454 bp) E. coli M718 genome assembly (5,444,046 bp)	LR890342.1 (100/100%) LN852838.1 (100/100%) OU349840.1 (86%/94.34%)	0.999281 identity Col(MG828)_1, NC_008486

Closest relatives were identified using the NCBI BLASTN suite and the megablast algorithm against the nr/nt database (Jan 16th 2022)
The hits shown were selected by sorting the best 100 hits by first accession length (ascending), then percent identity (descending) and finally query cover (descending)
The top three hits were selected. Plasmid replicon type was determined using PLSDB [39]

ISSN: 1476-0711