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Table 2 Conventional PCR primers

From: Rapid detection of a novel B1-β-lactamase gene, blaAFM-1 using a loop-mediated isothermal amplification (LAMP) assay

Gene

Primers (5′–3′)

Length (bp)

Annealing temperature (°C)

Recombinant plasmid AFM-1

pET-F: GATCCCGCGAAATTAATACG

1123

59.1

pET-R: GGCCCCAAGGGGTTATGCTAG

blaAFM-1

AFM-1-F: CGATTGGTGAGCAGGTGGATAAGG

336a

60.5

AFM-1-R: TCGACAAGGCATTGGCGTAAGTG

blaAFM-1 (full length)

AFM-F: ATGATTACGAAATCGAACATCGCG

804b

58.0

AFM-R: TCAGCGCAGCTTGTCGGC

  1. aFragment amplified by specific primers for blaAFM-1
  2. bFragment obtained from the full-length amplification of blaAFM-1