Gene | Primers (5′–3′) | Length (bp) | Annealing temperature (°C) |
---|---|---|---|
Recombinant plasmid AFM-1 | pET-F: GATCCCGCGAAATTAATACG | 1123 | 59.1 |
pET-R: GGCCCCAAGGGGTTATGCTAG | |||
blaAFM-1 | AFM-1-F: CGATTGGTGAGCAGGTGGATAAGG | 336a | 60.5 |
AFM-1-R: TCGACAAGGCATTGGCGTAAGTG | |||
blaAFM-1 (full length) | AFM-F: ATGATTACGAAATCGAACATCGCG | 804b | 58.0 |
AFM-R: TCAGCGCAGCTTGTCGGC |