Table 1 Optimal LAMP primer sets for blaAFM-1
Primer | Sequences (5′–3′) | Position& (bp) |
|---|---|---|
F3 | TTGGTGAGCAGGTGGATA | 95–112 |
B3 | AAGGTCAGGCTGTGCT | 479–494 |
FIP(F1c + F2) | TCATCGGTCCAGGCGGTAGGCAACATACCTCGTTCAT | – |
BIP(B1c + B2) | TCAGACCAGCCAGATCCTCAACCATCTTGTCCTGATGCG | – |
LF | CGCCATCCTTGACGATCA | 241–258 |
LB | CTGGATTAAGCAAGAGATCAATCTG | 300–324 |