Table 2 Primers used for virulence factors used in this study
Marker | Primer direction | Primer sequence (5′-3′) | Product length (bp) |
|---|---|---|---|
papA | Forward Reverse | ATGGCAGTGGTGTCTTTTGGTG CGTCCCACCATACGTGCTCTTC | 720 |
papC | Forward Reverse | GTGGCAGTATGAGTAATGACCGTTA ATATCCTTTCTGCAGGGATGCAATA | 200 |
papEF | Forward Reverse | GCAACAGCAACGCTGGTTGCATCAT AGAGAGAGCCACTCTTATACGGACA | 336 |
papG allele I | Forward Reverse | TCGTGCTCAGGTCCGGAATTT TGGCATCCCCCAACATTATCG | 461 |
papG allele II | Forward Reverse | GGGATGAGCGGGCCTTTGAT CGGGCCCCCAAGTAACTCG | 190 |
papG allele III | Forward Reverse | GGCCTGCAATGGATTTACCTGG CCACCAAATGACCATGCCAGAC | 258 |
sfa/focDE | Forward Reverse | CTCCGGAGAACTGGGTGCATFTTAC CGGAGGAGTAATTACAAACCTGGCA | 410 |
sfaS | Forward Reverse | GTGGATACGACGATTACTGTG CCGCCAGCATTCCCTGTATTC | 240 |
focG | Forward Reverse | CAGCACAGGCAGTGGATACGA GAATGTCGCCTGCCCATTGCT | 360 |
afa/draBC | Forward Reverse | GGCAGAGGGCCGGCAACAGGC CCCGTAACGCGCCAGCATCTC | 559 |
bmaE | Forward Reverse | ATGGCGCTAACTTGCCATGCTG AGGGGGACATATAGCCCCCTTC | 507 |
gafD | Forward Reverse | TGTTGGACCGTCTCAGGGCTC CTCCCGGAACTCGCTGTTACT | 952 |
nfaE | Forward Reverse | GCTTACTGATTCTGGGATGGA CGGTGGCCGAGTCATATGCCA | 559 |
fimH | Forward Reverse | TGCAGAACGGATAAGCCGTGG GCAGTCACCTGCCCTCCGGTA | 508 |
hlyA | Forward Reverse | AACAAGGATAAGCACTGTTCTGGCT ACCATATAAGCGGTCATTCCCGTCA | 1177 |
cnf1 | Forward Reverse | AAGATGGAGTTTCCTATGCAGGAG CATTCAGAGTCCTGCCCTCATTATT | 498 |
fyuA | Forward Reverse | TGATTAACCCCGCGACGGGAA CGCAGTAGGCACGATGTTGTA | 880 |
iutA | Forward Reverse | GGCTGGACATCATGGGAACTGG CGTCGGGAACGGGTAGAATCG | 300 |
kpsMT II | Forward Reverse | GCGCATTTGCTGATACTGTTG CATCCAGACGATAAGCATGAGCA | 272 |
kpsMT III | Forward Reverse | TCCTCTTGCTACTATTCCCCCT AGGCGTATCCATCCCTCCTAAC | 392 |
rfc | Forward Reverse | ATCCATCAGGAGGGGACTGGA AACCATACCAACCAATGCGAG | 788 |
ibeA | Forward Reverse | AGGCAGGTGTGCGCCGCGTAC TGGTGCTCCGGCAAACCATGC | 170 |
cvaC | Forward Reverse | CACACACAAACGGGAGCTGTT CTTCCCGCAGCATAGTTCCAT | 680 |
traT | Forward Reverse | GGTGTGGTGCGATGAGCACAG CACGGTTCAGCCATCCCTGAG | 290 |
PAI | Forward Reverse | GGACATCCTGTTACATCGCGCA TCGCCACCAATCACAGCCGAAC | 930 |