Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children

Table.1 Primer sequences, product sizes, and annealing temperatures for β-lactamase-encoding genes in clinical isolates of A. baumannii

Target gene	Primer sequence (5′-3′)	Product size (bp)	Annealing Temperature (°C)	References
bla _TEM	F: GAG TAT TCA ACA TTT CCG TG R: TAA TCA GTG AGG CAC CTA TC	848	47	[34]
bla _SHV	F: TTA TCT CCC TGT TAG CCA CC R: GAT TTG CTG ATT TCG CTC GG	797	52	[35]
bla _CTX-M	F: ACG CTG TTG TTA GGA AGT G R: TTG AGG CTG GGT GAA GT	759	52	[34]
bla _VEB	F: CGA CTT CCA TTT CCC GAT GC R: GGA CTC TGC AAC AAA TAC GC	643	54	[36]
bla _PER	F: GCA ACT GCT GCA ATA CTC GG R: ATG TGC GAC CAC AGT ACC AG	340	58	[37]
bla _GES	F: TTG CAA TGT GCT CAA CGT TC R: TAT CAC AAC CAA TAT TGT CG	630	48	[38]
bla _IMP	F: TTG ACA CTC CAT TTA CTG CTA R: TCA TTT GTT AAT TCA GAT GCA TA	172	50	[39]
bla _VIM	F: GAG TTG CTT TTG ATT GAT ACA G R: TCG ATG AGA GTC CTT CTA GA	247	51	[39]
bla _NDM	F: AAC ACA GCC TGA CTT TCG R: TGA TAT TGT CAC TGG TGT GG	111	53	[39]
ISAba1	F: CAC GAA TGC AGA AGT TG R: CGA CGA ATA CTA TGA CAC	549	48	[25]
bla _OXA-51-like	F: TAA TGC TTT GAT CGG CCT TG R: TGG ATT GCA CTT CAT CTT GG	353	53	[40]
bla _{OXA−24−like}	F: GGT TAG TTG GCC CCC TTA AA R: AGT TGA GCG AAA AGG GGA T	246	54	[40]
bla _OXA-23-like	F: GAT CGG ATT GGA GAA CCA GA R: ATT TCT GAC CGC ATT TCC AT	501	52	[40]
bla _OXA-58-like	F: AAG TAT TGG GGC TTG TGC TG R: CCC CTC TGC GCT CTA CAT AC	599	56	[40]
ISAba1/bla_OXA-23-like	F: CAC GAA TGC AGA AGT TG R: ATT TCT GAC CGC ATT TCC AT	1404	50	[40]
ISAba1/bla_OXA-51-like	F: CAC GAA TGC AGA AGT TG R: TGG ATT GCA CTT CAT CTT GG	1223	52	[40]

ISSN: 1476-0711