Table 1 Primers, product sizes, and annealing temperatures used for this study
Primer name | Primer sequence (5´ → 3´) | Amplicon size (bp) | Annealing temperature (ºC) | Refs. |
|---|---|---|---|---|
iucA | F: AATCAATGGCTATTCCCGCTG R: CGCTTCACTTCTTTCACTGACAGG | 239 | 62 | [18] |
peg-344 | F: GCGGGAAAGGACAGAAAGCCAGTG R: GAGGGAAGATGAGAAATACGAGC | 332 | 56 | This study |
iutA | F: GCCGCTAGGTTGGTGATGT R: CTCTGGTCGTGCTGGTTGA | 949 | 61 | This study |
iroB | F: GTGTTGGATTCCGCCAGTGA R: TTCCGCCGCTACCTCTTCA | 366 | 61 | This study |
magA | F: GGTGCTCTTTACATCATTGC R: GCAATGGCCATTTGCGTTAG | 1282 | 51 | [47] |
rmpA | F: GAGTATTGGTTGACAGCAGGAT R: AGCCGTGGATAATGGTTTACAA | 250 | 53 | This study |
kfu | F: ATAGTAGGCGAGCACCGAGA R: AGAACCTTCCTCGCTGAACA | 520 | 60 | [48] |
allS | F: CCGAAACATTACGCACCTTT R: ATCACGAAGAGCCAGGTCAC | 508 | 60 | [48] |
ybt | F: GACGGAAACAGCACGGTAAA R: GAGCATAATAAGGCGAAAGA | 242 | 60 | [47] |
blaCTX-M-15 | F: CGCTTTGCGATGTGCAG R: ACCGCGATATCGTTGGT | 590 | 53 | This study |
blaTEM | F: GAGTATTCAACATTTCCGTGTC R: TAATCAGTGAGGCACCTATCTC | 800 | 54 | [49] |
blaSHV | F: AAGATCCACTATCGCCAGCAG R: ATTCAGTTCCGTTTCCCAGCGG | 200 | 60 | [49] |
blaOXA-48 | F-GCGTGGTTAAGGATGAACAC R-CATCAAGTTCAACCCAACCG | 745 | 60 | [49] |
blaKPC-1 | F: CGTCTAGTTCTGCTGTCTTG R:CTTGTCATCCTTGTTAGGCG | 798 | 55 | [50] |
blaNDM-1 | F: GGTTTGGCGATCTGGTTTTC R: CGGAATGGCTCATCACGATC | 621 | 54 | [50] |
ompk36 group A | F: GAAGGCGCTCTGTCTCCTA R: TGCCATCATAGATGTCATAGG | 97 | 60 | [13] |
ompk36 group B | F: CGGTCGTGGCGCGCAGAAA R: GGTTGTTCTGA TCGTCGGTA | 125 | 64 | [13] |
ompk36 group C | F: CAACAACGGTCGT GGTTGGA R: CCCAGTGCCGGAACACTATT | 144 | 62 | [13] |
ompk36 group D | F: GAAGGTACTTCTCCGACCAA R: AATCAGATTCTCCGTTGCCG | 283 | 62 | [13] |