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Table 4 Determination of biofilm formation in CNS by genotypic and phenotypic methods

From: Phenotypic and genotypic characterization of biofilm producing clinical coagulase negative staphylococci from Nepal and their antibiotic susceptibility pattern

Biofilm CNS isolates Total (n = 214)
S. epidermidis S. saprophyticus S. haemolyticus S. hominis S. capitis
(n = 123) (n = 40) (n = 24) (n = 15) (n = 12)
Congo red agar (CRA)
 Strong 12 (9.8%) 2 (5.0%) 3 (12.5%) 3 (20.0%) - 20 (9.3%)
 Moderate 12 (9.8%) 2 (5.0%) 5 (20.8%) 2 (13.3%) 2 (16.7%) 23 (10.7%)
 Weak/none 99 (80.5%) 36 (90.0%) 16 (66.7%) 10 (66.7%) 10 (83.3%) 171 (79.9%)
Tube adherence method (TM)
 Strong 33 (26.8%) 4 (10.0%) 2 (8.33%) 3 (20.0%) 2 (16.7%) 44 (20.6%)
 Moderate 9 (7.3%) 6 (15.0%) 6 (25.0%) 3 (20.0%) - 24 (11.2%)
 Weak/none 81 (65.9%) 30 (75.0%) 16 (66.7%) 9 (60.0%) 10 (83.3%) 146 (68.2%)
Tissue culture plate (TCP)
 Strong 23 (18.7%) 8 (20.0%) 3 (12.5%) 1 (6.7%) - 35 (16.4%)
 Moderate 32 (26.0%) 9 (22.5%) 4 (16.7%) 7 (46.7%) 3 (25.0%) 55 (25.7%)
 Weak/none 68 (55.3%) 23 (57.5%) 17 (70.8%) 7 (46.7%) 9 (75.0%) 124 (57.9%)
ica gene
 Present 29 (23.6%) 5 (12.5%) 2 (8.33%) 3 (20.0%) 2 (16.7%) 41 (19.2%)
 Absent 94 (76.4%) 35 (87.5%) 22 (91.7%) 12 (80.0%) 10 (83.3%) 173 (80.8%)