Table 1 Parameters for each of the six nucleic acid amplification kits evaluated
From: Comparative evaluation of six nucleic acid amplification kits for SARS-CoV-2 RNA detection
Kit | Target | Volume (uL) | Results interpretation | LOD (copies/mL) | Ref | ||
|---|---|---|---|---|---|---|---|
sample | RNA | Positive | Negative | ||||
A | ORF 1ab, N | 500 | / | Tt a ≤ 40 | No Tt value | 1000 | Y |
B | ORF 1ab, N | / | 5 | Ct ≤ 38 | No Ct value or Ct > 38 | 1000 | N |
C | ORF 1ab, N | / | 5 | Ct ≤ 35 | Ct > 38 | 500 | N |
D | ORF 1ab | / | 10 | Ct ≤ 32 | FAM: No Ct value VIC/HEX: Ct ≤ 32 | 100 | Y |
E | ORF 1ab, N | / | 4 | Ct < 38 | No Ct value or Ct = 45 | 300 | N |
F | ORF 1ab, N | / | 5 | Ct ≤ 40 | No Ct value or Ct > 40 | 500 | Y |