Characterization of antimicrobial susceptibility, extended-spectrum β-lactamase genes and phylogenetic groups of Shigatoxin producing Escherichia coli isolated from patients with diarrhea in Iran

Table 1 Primer sequences and sizes of PCR products

Target GENE	Amplicon size (bp)	Primer Sequence (5′ to 3′)	References
Virulence genes
Stx1A	244	F: CGATGTTACGGTTTGTTACTGTGACAGC	[13]
Stx1A	244	R: AATGCCACGCTTCCCAGAATTG	[13]
Stx2A	324	F: GTTTTGACCATCTTCGTCTGATTATTGAG	[13]
Stx2A	324	R: AGCGTAAGGCTTCTGCTGTGAC	[13]
eae	570	F: AGGCTTCGTCACAGTTG	[14]
eae	570	R: CCATCGTCACCAGAGGA	[14]
β-lactamase genes
blaCTX-M9	856	F: GTGACAAAGAGAGTGCAACGG	[17]
blaCTX-M9	856	R: ATGATTCTCGCCGCTGAAGCC	[17]
blaSHV	768	F: TCGCCTGTGTATTATCTCCC	[18]
blaSHV	768	R: CGCAGATAAATCACCACAATG	[18]
blaOXA	438	F: GCGTGGTTAAGGATGAACAC	[19]
blaOXA	438	R: CATCAAGTTCAACCCAACCG	[19]
blaTEM	963	F: GCGGAACCCCTATTTG	[20]
blaTEM	963	R: ACCAATGCTTAATCAGTGAG	[20]
Phylogenetic genes
chuA^a	288	F: ATGGTACCGGACGAACCAAC	[8]
chuA^a	288	R: TGCCGCCAGTACCAAAGACA	[8]
yjaA^a	211	F: CAAACGTGAAGTGTCAGGAG	[8]
yjaA^a	211	R: AATGCGTTCCTCAACCTGTG	[8]
arpA^a	400	F: AACGCTATTCGCCAGCTTGC	[8]
arpA^a	400	R: TCTCCCCATACCGTACGCTA	[8]
TspE4.C2^a	152	F: CACTATTCGTAAGGTCATCC	[8]
TspE4.C2^a	152	R: AGTTTATCGCTGCGGGTCGC	[8]
arpA__gpE	301	F: GATTCCATCTTGTCAAAATATGCC	[8]
arpA__gpE	301	R: GAAAAGAAAAAGAATTCCCAAGAG	[8]
trpA__gpC	219	F: AGTTTTATGCCCAGTGCGAG	[8]
trpA__gpC	219	R: TCTGCGCCGGTCACGCCC	[8]
trpA__{Internal control}	489	F: CGGCGATAAAGACATCTTCAC	[8]
trpA__{Internal control}	489	R: GCAACGCGGCCTGGCGGAAG	[8]

ISSN: 1476-0711