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Table 2 Lists primers for species specific (P. aeruginosa), efflux pumps and biofilm forming genes used in this study

From: Evaluation of efflux pump activity and biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa isolated from a Federal Medical Center in Nigeria

Primer Sequence Product size (bp) Annealing temperature (°C) Reference
mexA-F
mexA-R
5′-ACCTACGAGGCCGACTACCAGA-3′
5′-GTTGGTCACCAGGGCGCCTTC-3′
179 57 [20]
mexB-F
mexB-R
5′-GTGTTCGGCTCGCAGTACTC-3′
5′-AACCGTCGGGATTGACCTTG-3
244 56 [20]
oprM-F
oprM-R
5′-CCATGAGCCGCCAACTGTC-3′
5′-CCTGGAACGCCGTCTGGAT-3′
205 57 [20]
pslA-F
pslA-R
5′-TGGGTCTTCAAGTTCCGCTC-3′
5′-ATGCTGGTCTTGCGGATGAA-3′
119 55 [21]
pslD-F
pslD-R
5′-CTCATGAAACGCACCCTCCT-3′
5′-TGCGACCGATGAACGGATAG-3′
295 52 [21]
PA-SSF
PA-SSR
5′-GGGGATCTTCGGACCTCA-3′
5′-TCCTTAGAGTGCCCACCCG-3′
956 56 [22]
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