Skip to main content

Table 1 Performance of the different rapid identification methods using MALDI-TOF–MS on positive blood cultures

From: Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS

References Methods Sensibility (%) Estimated technical time
Commercial kits
 [44] Sepsityper kit® (Bruker Daltonics) 75.6% (n = 160) 35 min (5 centrifugation steps)
 [45] 80.8% (n = 411)
 [30] 79.8% (n = 3320)
 [46] Vitek MS Blood culture kit® (Biomérieux) 73% (n = 259) 15 min (No centrifugation steps)
 [47] Rapid BACpro®II kit (Nittobo Medical Co) 76.5% (n = 17) 15 min (4 centrifugation steps)
 [43] Rapid BACpro®II kit (Nittobo Medical Co) improvement 96,3% (n = 269)
 This study Rapid Sepsityper® protocol (Bruker Daltonics): RS ± FA
Complete Sepsityper® protocol on unidentified samples (n = 94)
68.6% (n = 299)
78.6% (n = 299)
10 min (2 centrifugation steps)
35 min (5 centrifugation steps)
In-house protocols: centrifugation
 [48] Centrifugation 95% (n = 277)  > 20 min (> 5 centrifugation steps)
 [41] Centrifugation 43% (n = 79)  > 10 min (4 centrifugation steps)
 [49] Centrifugation 85.9% (n = 85) 15 min (2 centrifugation steps)
In-house protocols: centrifugation in Separator tube
 [50] Clot activator and gel BD Vacutainer tubes® (BD Diagnostics) ACUETTE® Z Serum Sept Clot Activator (Greiner Bio-One) 89.6% (n = 532)  > 20 min (5 centrifugation steps)
 [51] ACUETTE® Z Serum Sept Clot Activator (Greiner Bio-One) 90% (n = 186) 15 min (2 centrifugation steps)
 [42] Separator tube with plasma separation gel 88.7% (n = 789)  > 20 min (2 centrifugation steps)
 [52] Serum Separator tube (BD Diagnostics) 68.7% (n = 195)  > 30 min (5 centrifugation steps)
In-house protocols: centrifugation + lysis reagent
 [53] 5% Saponin lysis (fast protocol)
20% SDS lysis (fast protocol)
53% (n = 42)
86% (n = 42)
20 min (2 centrifugation steps)
 [54] 0.6% polyoxyethylene 10 oleoyl ether (Brij 97) in 0.4 mol/L 3-cyclohexylamino-1-propane sulfonic acid lysis 82,4% (n = 125)  > 10 min (2 centrifugation steps)
 [34] Triton X-100 lysis 80.5% (n = 681) 10 min (2 centrifugation steps)
In-house protocols: Centrifugation + lysis reagent + additional protein extraction
 [55] Saponin lysis
 + 10% TFA extraction
61.4% (n = 127) 20 min (2 centrifugation steps)
 [56] 5% Saponin lysis
 + Ethanol/FA extraction
80.6% (n = 155) 20 min (2 centrifugation steps)
 [57] 5% Saponin lysis
10% SDS lysis
 + 100% Ethanol/100% Acetonitrile/70% FA extraction
79.5% (n = 176) 88% (n = 176)  > 20 min (5 centrifugation steps)
 [41] 10% SDS lysis
 + 70% FA/Acetonitrile extraction
49% (n = 101)  > 20 min (4 centrifugation steps)
 [58] Ammonium chloride lysis
 + 70% Ethanol/70% FA extraction
78.7% (n = 122) 30–45 min (4 centrifugation steps)
 [59] 0.1% TFA lysis
 + 70% FA/100% Acetonitrile extraction
88.9% (n = 245)  > 40 min (6 centrifugation steps)
 [40] 0.2% Triton X-100 + 0.1% SDS lysis
0.2% Triton X-100 + 1.8% SDS lysis
 + Ethanol/100% Acetonitrile/70% FA extraction + 70% FA extraction
86.3% (n = 450)
86.4% (n = 59)
30 min (5 centrifugation steps)
10 min (3 centrifugation steps)
  1. The technical time is either directly issued from publications when available or estimated from the number of centrifugation and centrifugation time described in protocols (estimated as > to x min with centrifugation steps between 1 to 3 min). (SDS: Sodium dodecyl sulfate; FA, formic acid; TFA, trifluoroacetic acid; n, number of samples tested)