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Fig. 5 | Annals of Clinical Microbiology and Antimicrobials

Fig. 5

From: Differential effects of antibiotics on neutrophils exposed to lipoteichoic acid derived from Staphylococcus aureus

Fig. 5

Effects of high (10 μg) and low (1 μg) levels of LTA and antibiotics on PMN lifespan (a) and survival (b) in human neutrophils isolated from healthy volunteers (n = 5). Effects on PMN lifespan and survival were determined at 16 h following stimulation using flow cytometry with Annexin V and propidium iodide (PI). Live cells are unstained, whereas Annexin V stains apoptotic cells and PI permeates dead cells. Double-stained cells are considered necrotic. Representative scatter plots of unstimulated cells and cells exposed to high LTA with and without antibiotics are shown in c. Statistical significance was determined using two way ANOVA * p < 0.05, **p < 0.01. VAN, vancomycin; CFT, ceftaroline; DAP, daptomycin

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