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Table 1 The forward and reverse primers used in pgaA and pgaC genes

From: Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal

Gene

Primers

Sequences

GC %

Tm (°C)

pgaA

Forward

5′-GGCTTTGAAACTTCTTACTGC-3′

42.9

57.4

Reverse

5′-CCTGTTTATCTTGCCCGGCC-3′

60

62.5

pgaC

Forward

5′-ATGATTAATCGCATCGTATCG-3′

38.1

55.5

Reverse

5′-CATCGGTTCCACAATATATGC-3′

42.9

57.4