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Table 3 Determination of Salmonella serovars using O and H antigens identified from the two-tube MLMA system

From: Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars

No.

Salmonella serovars

O antigen

H1 antigen

H2 antigen

1

Typhimurium

1-ROX-70.5-O:4 (B)

2-ROX-59-H1-i

1-CY5-55.5-H2-(1,2)

2

Enteritidis

1-ROX-54.5-O:9 (D)

2-ROX-54-H1-(g,m)

3

Paratyphi A

1-ROX-67-O:2 (A)

1-FAM-54.5-H1-a

4

Typhi

1-ROX-54.5-O:9 (D)

2-FAM-74.5-H1-d

5

Stanley

1-ROX-70.5-O:4 (B)

2-FAM-74.5-H1-d

1-CY5-55.5-H2-(1,2)

6

London

1-ROX-74.5-O:3 (E)

2-ROX-57-H1-v

2-CY5-50.5-H2-(1,6)

7

Derby

1-ROX-70.5-O:4 (B)

2-FAM-59-H1-f,g

8

Senftenberg

1-ROX-74.5-O:3 (E)

1-FAM-50.5-H1-(f,g,s/g,s,t)

9

Agona

1-ROX-70.5-O:4 (B)

1-FAM-50.5-H1-(f,g,s/g,s,t)

10

Weltevreden

1-ROX-74.5-O:3 (E)

2-ROX-62.5-H1-r

1-CY5-50.5-H2-z6

11

Anatum

1-ROX-74.5-O:3 (E)

2-FAM-64-H1-(e,h)

2-CY5-50.5-H2-(1,6)

12

Choleraesuis

1-ROX-64-O:7 (C1)

2-FAM-52.5-H1-c

2-CY5-57-H2-(1,5)

13

Infantis

1-ROX-64-O:7 (C1)

2-ROX-62.5-H1-r

2-CY5-57-H2-(1,5)

14

Muenchen

1-ROX-59-O:8 (C2-C3)

2-FAM-74.5-H1-d

1-CY5-55.5-H2-(1,2)

15

Braenderup

1-ROX-64-O:7 (C1)

2-FAM-64-H1-(e,h)

2-ROX-74-H2-(e,n,x/e,n,z15)

16

Papuana

1-ROX-64-O:7 (C1)

2-ROX-62.5-H1-r

2-ROX-74-H2-(e,n,x/e,n,z15)

17

Chennai

1-ROX-70.5-O:4 (B)

2-FAM-74.5-H1-d

18

Rissen

1-ROX-64-O:7 (C1)

1-FAM-50.5-H1-(f,g,s/g,s,t)

19

Fillmore

1-ROX-59-O:8 (C2-C3)

2-FAM-64-H1-(e,h)

2-ROX-74-H2-(e,n,x/e,n,z15)

20

Virchow

1-ROX-64-O:7 (C1)

2-ROX-62.5-H1-r

1-CY5-55.5-H2-(1,2)

21

Saintpaul

1-ROX-70.5-O:4 (B)

2-FAM-64-H1-(e,h)

1-CY5-55.5-H2-(1,2)

22

Litchfield

1-ROX-59-O:8 (C2-C3)

2-ROX-57-H1-v

1-CY5-55.5-H2-(1,2)

23

Corvallis

1-ROX-59-O:8 (C2-C3)

1-FAM-75.5-H1-(z4,z23)

24

Chester

1-ROX-70.5-O:4 (B)

2-FAM-64-H1-(e,h)

2-ROX-74-H2-(e,n,x/e,n,z15)

25

Heidelberg

1-ROX-70.5-O:4 (B)

2-ROX-62.5-H1-r

1-CY5-55.5-H2-(1,2)

26

Give

1-ROX-74.5-O:3 (E)

2-ROX-57-H1-v

1-CY5-63.5-H2-(1,7)

27

Lagos

1-ROX-70.5-O:4 (B)

2-ROX-59-H1-i

2-CY5-57-H2-(1,5)

28

Essen

1-ROX-70.5-O:4 (B)

2-ROX-54-H1-(g,m)

29

Nigeria

1-ROX-64-O:7 (C1)

2-ROX-62.5-H1-r

2-CY5-50.5-H2-(1,6)

30

Paratyphi B

1-ROX-70.5-O:4 (B)

1-FAM-62-H1-b

1-CY5-55.5-H2-(1,2)

  1. The table below illustrates the experimental setup for the detection of each specific antigen in the assay, including the tube number, the fluorophore channel, the designated melting temperature (Tm, °C) and the antigen being detected. As an example, the detection of O antigen in S. Typhimurium is represented by “1-ROX-70.5-O:4 (B)”, where “1” represents the first tube, “ROX” indicates the fluorophore channel, 70.5 indicates the melting peak temperature (°C), O:4 (B) signifies the O antigen group designation