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Table 2 Identification matrix of gene targets in a two-tube MLMA system

From: Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars

First tube

Antigen

Serogroup/

factors

Tm(°C)

Second

tube

Antigen

Serogroup/

factors

Tm(°C)

ROX channel

O

E

74.5

ROX channel

H2

(e,n,x/e,n,z15)

74.0

B

70.5

H1

r

62.5

A

67.0

i

59.0

C1

64.0

v

57.0

C2-C3

59.0

(g,m)

54.0

D1

54.5

  

FAM channel

H1

(z4,z23)

75.5

FAM channel

H1

d

74.5

ssaR

b

66.0

61.0

(e,h)

64.0

a

54.5

(f,g)

59.0

(f,g,s/g,s,t)

50.5

c

52.5

Cy5 channel

H2

(1,7)

61.5

Cy5 channel

H2

D2

62.0

(1,2)

55.5

(1,5)

57.0

z6

50.5

(1,6)

50.5