Fig. 4

Photomicrographs of spleen; A Normal architecture of spleen from a healthy rat showing regular red and white pulps with central artery (arrow) and distinct germinal center (asterisk); B treated by free SLN: Inflammation of the organ with granulomatous reaction (arrow) and subcapsular congestion (asterisk); C Untreated. Diffuse inflammation and congestion of the organ with hemosiderin precipitation (arrowheads); D treated by DOX-SLN. Microscopic architecture of spleen is being restored with typical red pulps (black asterisk) and white pulps (white asterisk) with marginal zone (circle); E treated by free Doxycycline. The organ architecture is being regenerated with typical red and white pulps. However, occasional granulomatous reaction exists in the red pulp (arrow). Hematoxylin & Eosin, Magnification = ×400