Fig. 5

Histological images of bone sections harvested at 28 dpi and stained with Masson Goldner’s trichrome. a Group 1, group 2 and group 5 showing mild periosteal reaction consisting of normal bone architecture; slight distribution of immature connective tissue (CT), numerous active harversian canals (H), endosteum-layer (E) and marrow cavity. b Bone sections of groups 3 and group 4 showing osteomyelitis consisting of progressive destruction of bone architecture, mineral component of the bone and no bone formation at the defect site (D), note the bacterial colony (BC), empty lacunae (L) and scattered sequestra (S). ×40 magnification. c Quantification of histological images of experimental rabbits (groups 1–5) respectively, after 28 days follow up. Error bar represent the standard error of mean. *p < 0.05. d Quantification of histological images of experimental rabbits (groups 1–5) after 28 days follow-up. Confirmation of new bone formation and microanatomical changes. ×40 magnification