|
Strain
|
Adherence ratea
|
Invasion rateb
|
Survival within macrophagesc
|
Biofilm formation rated
|
|---|
|
C7223
|
60.4 b/c
|
1.8 × 102
|
3.3 × 102
|
48.13
|
|
C7225
|
17.3 b/c
|
1.2 × 108
|
2.3 × 105
|
51.44
|
|
C7236
|
18.9 b/c
|
1.4 × 108
|
2.0 × 105
|
46.9
|
|
C7230
|
12.6 b/c
|
3.2 × 104
|
4.4 × 103
|
46.47
|
- Determination of E. coli strains as belonging to the AIEC pathotype was performed using the following criteria: (1) the ability to adhere to HeLa cells with an adhesion index equal or superior to 1 bacteria per cell (b/c), (2) the ability of the bacteria to invade HeLa cells with an invasion index equal or superior to 0.1% of the original inoculum, (3) the ability to survive and to replicate within J774 macrophages
- aAdherence rate is expressed as the mean of bacteria adhered to one HeLa cell
- bInvasion is the mean CFUs after 1 h rifampicin treatment of infected HeLa cells
- cMean of intracellular bacteria at 3 h post infection relative to the number after 1 h rifampicin treatment, defined as 100% (MOI of 100)
- dBiofilm formation rate is defined as milligrams per grams of total protein
