Table 2 Specific primers used in this study
Target | Primer name | Sequence 5′ to 3′ | Tm ( °C) | Amplicon size | Reference |
|---|---|---|---|---|---|
PAI-ICFT073 | RPAi | GGACATCCTGTTACAGCGCGCA | 65 | 925 bp | [68] |
RPAf | TCGCCACCAATCACAGCGAAC | ||||
PAI-IICFT073 | Cft073.2Ent1 | ATGGATGTTGTATCGCGC | 55 | 400 bp | [69] |
cft073.2Ent2 | ACGAGCATGTGGATCTGC | ||||
PAI-IJ96 | PapGIf | TCGTGCTCAGGTCCGGAATTT | 57.7 | 400 bp | [68] |
PapGIr | TGGCATCCCACATTATCG | ||||
PAI-IIJ96 | Hlyd | GGATCCATGAAAACATGGTTAATGGG | 59.3 | 2.3 kb | [70] |
Cnf | GATATTTTTGTTGCCATTGGTTACC | ||||
fimH | FimH-F | CACTCAGGGAACCATTCAGGCA | 57 | 975 bp | [71] |
FimH-R | CTTATTGATAAACAAAAGTCAC | ||||
Iron | IRON-F | AAGTCAAAGCAGGGGTTGCCCG | 60 | 667 bp | [16] |
IRON-R | GACGCCGACATTAAGACGCAG | ||||
afa operon | AFA025-F | GAGTCACGGCAGTCGCGGCGG | 55 | 207 bp | [72] |
AFA025-R | TTCACCGGCGACCAGCCATCTCC | ||||
afa/draBC | Afa-DraF | GGCAGAGGGCCGGCAACAGGC | 60 | 559 bp | [68] |
Afa-DraR | CCCGTAACGCGCCAGCATCTC | ||||
ibeA | IBEA 10 F | AGGCAGGTGTGCGCCGCGTAC | 60 | 170 bp | [68] |
IBEA 10 R | TGGTGCTCCGGCAAACCATGC | ||||
cnfI | cnf-f | ATCTTATACTGGATGGGATCATCTTGG | 60 | 974 bp | [73] |
cnf-r | GCAGAACGACGTTCTTCATAAGTAT | ||||
cdtB | cdtB-f | AACTGATTTTCGCGTTGCGA | 60 | 741 bp | This study |
cdtB-r | GATACGCCAACAGGGAAATG | ||||
neuC-KI | kpsII-f | GATACGCCAACAGGGAAATG | 63 | 272 bp | [68] |
kpsII-r | CATCCAGACGATAAGCATGAGCA | ||||
KI-f | TAGCAAACGTTCTATTGGTGC | 153 bp | |||
insA | insA-f | GGCATCCAACGCCATTCAT | 62 | 178 bp | This study |
insA-r | TGTCCCTCCTGTTCAGCTACTGA | ||||
insB | insB-f | ATGTTCAGATAATGCCCGATG | 62 | 461 bp | This study |
insB-r | CGTTGGCCTCAACACGATTT | ||||
vatA | vatA1076F | CCTGGGACATAATGGTCAGAT | 61 | 330 bp | Arenas-Hernández unpublished data |
vatA1406R | CTGGCAATATTCACGCTACTG | ||||
vatP | vatP-86F | TAGCGCGCAATTCAACAATA | 61 | 226 bp | Arenas-Hernández unpublished data |
vatP226R | GCAGATAGTGCCAGAGAGGTAAG | ||||
intI1 | IntI1-F | GGGTCAAGGATCTGGATTTCG | 62 | 483 bp | [74] |
IntI1-R | CGACGATGATTTACACGCATGT | ||||
papa | papA-45F | CAGATATCTCGGTGTGTTCAGTAA | 61 | 641 bp | Arenas-Hernández unpublished data |
papA + 31R | GGTCTTGCCTCACCCTGTAA | ||||
iha | ihaEMSAR | CGGAATTCCGATCTCCGATCATGTTAACCG | 61 | 150 bp | [75] |
ihaEMSAL | CGGAATTCCGGCATGCCGAGGCAGTCGTTA | ||||
iucD | iucD-30F | GCTGTGGCTGGTAACTCAGG | 58 | 512 bp | Arenas-Hernández unpublished data |
iucD512R | TGCTTCACACAGGGTGGTAAAT | ||||
fliC | FliC 242F | GCTGTCCGAAATCAACAACAA | 58 | 304 bp | Arenas-Hernández unpublished data |
FliC 445 R | GGCTATCGTACCGGAACCATT | ||||
Fimbrial adhesin subunit | daaE-F | TGACTGTGACCGAAGAGTGC | 48 | 380 bp | [76] |
daaE-R | TTAGTTCGTCCAGTAACCCCC | ||||
IS3 Transposase family | STI-F | TTAATAGCACCCGGTACAAGCAGG | 64 | 147 bp | [77] |
STI-R | CTTGACTTCTTCAAAAGAGAAAATTAC | ||||
Heat-stable enterotoxin | STaII-F | TTGTCTTTTTCACCTTTCCC | 60 | 93 bp | [78] |
STaII-R | ACAAGCAGGATTACAACACA | ||||
Heat-labile enterotoxin | LT-F | GGCGACAGATTATACCGTGC | 60 | 750 bp | [79] |
LT-R | CCGAATTCTGTTATAATATGTC | ||||
Intimin | eae-F | CAGGTCGTCGTGTCTGCTAAA | 67 | 1087 bp | [80] |
eae-R | TCAGCGTGGTTGGATCAACCT | ||||
Shiga toxin 1 | STx1-F | TTTACGATAGACTTCTCGAC | 55 | 227 bp | [81] |
STx2-R | CACATATAAATTATTTCGCTC | ||||
Shiga toxin 2 | STx2-F | CCCAGTCACGACGTTGTA | 60 | 460 bp | [78] |
STx2-R | TATACTATCGTGCCTTTCCA | ||||
ial | ial-F | CTGGATGGTATGGTGAGG | 60 | 320 bp | [82] |
ial-R | GGAGGCCAACAATTATTTCC | ||||
EAF | EAF-F | CAGGGTAAAAGAAAGATGATAA | 58 | 1087 bp | [83] |
EAF-R | TATGGGGACCATGTATTATCA | ||||
bfpA | bfpA-F | AATGGTGCTTGCGCTTGCTGC | 67 | 326 bp | [79] |
bfpA-R | GCCGCTTTATCCAACCTGGTA | ||||
EAEC plasmid | EAEC-F | CTGGCGAAAGACTGTATCAT | 60 | 630 bp | [84] |
EAEC-R | CAATGTATAGAAATCGCTGTT |