Table 1 PCR primers characteristics in this study
PCR target | Primer name | Primer sequence (5′→3′) | Annealing temperature (°C) | Product (bp) | Reference |
|---|---|---|---|---|---|
Upstream flanking region of blaCTX-M-55 | ISEcp1-F | CAAAATGATCCCCTCGTCAAC | 55 | Variable | [29] |
IS26-F | TTACATTTCAAAAACTCTGCTTACC | 57 | Variable | [32] | |
blaCTX-M-1-R | ACTTTACTGGTACTGCACAT | Â | Â | Â | |
Downstream flanking region of blaCTX-M-55 | blaCTX-M-1-F | TTCTGGTGACRTACTTRACCCA | Â | Â | Â |
IS903-R | GTTTAATGACCAGCACAGT | 55 | 364 | This study | |
ORF477-R | TCGTTTCGTGGTGCTGAATTT | 57 | Variable | [29] | |
bla CTX-M-1 | blaCTX-M-1-F | CAGCGCTTTTGCCGTCTAAG | 52 | 946 | This study |
blaCTX-M-1-R | GGCCCATGGTTAAAAAATCACTGC | ||||
bla TEM | bla TEM -F | CATTTCCGTGTCGCCCTTATTC | 56 | 800 | [11] |
bla TEM -R | CGTTCATCCATAGTTGCCTGAC | ||||
bla SHV | bla SHV -F | AGCCGCTTGAGCAAATTAAAC | 55 | 713 | [11] |
bla SHV -R | ATCCCGCAGATAAATCACCAC |