Benzoylphenyl thiocyanates are new, effective inhibitors of the mycobacterial resuscitation promoting factor B protein

Table 1 Inhibitory effect of the NPT and BPT compounds on the Rpf-mediated hydrolysis of mycobacterial peptidoglycan and on bacterial growth

№	Chemical name	Formula	IC₅₀ Rpf-hydrolysis of PG, µM		MIC of the compound, µM
№	Chemical name	Formula	M. smegmatis	M. tuberculosis	M. luteus	M. smegmatis
(I)	4-Benzoyl-2-nitrophenyl thiocyanate		2.4 ± 0.7	4.0 ± 2.1	25–35	18–35
(II)	3-Benzoylphenyl thiocyanate		0.4 ± 0.0	1.7 ± 0.4	32–42	32–42
(III)	4-Benzoylphenyl thiocyanate		0.9 ± 0.5	1.2 ± 0.5	25–32	21–42
(IV)	Bis(4-thiocyano-3-nitrophenyl)methanone		3.7 ± 0.2	5.8 ± 2.0	47–52	26–52
(V)	Bis(4-thiocyanophenyl)methanone		2.5 ± 0.6	4.3 ± 2.4	48–51	34
(VI)	Bis(3-thiocyanophenyl)methanone		2.0 ± 0.8	3.6 ± 1.2	34–41	34

Enzymatic activity was calculated as a value of the half maximal inhibitory concentration (IC₅₀). IC₅₀ values were calculated in the enzymatic assay of the Rpf-mediated PG hydrolysis (concentration of RpfB_280–326 was 10 μg/ml). The experiment was repeated three times with nearly identical results (mean values ± SD are shown)
MIC was evaluated as the minimal inhibitory concentration of the compounds sufficient for suppression of active growth of the bacteria. The experiments were repeated at least three times with nearly identical results (mean values ± SD are shown)

ISSN: 1476-0711