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Table 3 Antibiotic-resistance profile of MDR strains and AgNPs susceptibility test

From: Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread

Bacteria

Tested antibiotics

Disk content (µg)

ZOI (mm)

Antibiotics susceptibility interpretationa

ESBL+ve E. coli

Nalidixic acid

30

6.0 ± 0.50

R

Doxycycline

30

16.16 ± 0.28

S

Norfloxacin

10

8.0 ± 0.50

R

Nitrofurantoin

30

15.0 ± 0.50

I

Cefixime

30

6.0 ± 0.50

R

Cefixime/clavulanate

5/10

8.0 ± 0.50

R

Cefoperazone

75

6.0 ± 0.0

R

Cefoperazone/tazobactam

75/10

19.5 ± 1.0

S

Ceftazidime

30

7.0 ± 1.0

R

Cetazidime/clavulanate

30/10

14.0 ± 0.0

I

Chloramphenicol

30

15.16 ± 0.28

I

Cefprozil

30

6.5 ± 0.50

R

Cefepime

30

10.5 ± 0.50

R

AgNPs

2.16b

10.5 ± 0.50

–

AgNO3

3.4

9.16 ± 0.28

–

Sterile H2O

20 µl

0 ± 0

–

S. aureus (MRSA)

Oxacillin

1

6.66 ± 0.28

R

Cefoxitin

30

19.33 ± 0.57

R

AgNPs

2.16b

13.5 ± 0.5

–

AgNO3

3.4

11.0 ± 0.0

–

Sterile H2O

20 µl

0 ± 0

–

S. pneumoniae MDR

Teicoplanin

30

10.0 ± 0.0

R

AgNPs

2.16b

13.16 ± 0.28

–

AgNO3

3.4

11.0 ± 0.0

–

Sterile H2O

20 µl

0 ± 0

–

  1. All experiments were performed in triplicate
  2. R resistant, I intermediate, S susceptible
  3. aThe antibiotics susceptibility interpretation was based on CLSI guideline-2016
  4. b20 μl of 1 mM AgNO3 (MW 169.87) contains 3.4 μg AgNO3 and the percentage of silver is 63.5% in nanoparticles (i. e 2.16 μg)