Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread

Table 2 Antimicrobial activity of colloidal AgNPs against various species of pathogenic bacteria, yeast and molds

Sr. no.	Microorganisms	ZOI of 20 μl of AgNPs (mm) (y)	ZOI of 20 μl of 1 mM AgNO₃ (mm) (x)	ZOI of 20 μl of H₂O (mm)	Increase in the ZOI area (%)^a
1	E. coli	12.66 ± 0.28	11.00 ± 0.0	0 ± 0	32.45
2	S. pneumoniae	14.33 ± 0.57	13.16 ± 0.28	0 ± 0	18.57
3	K. pneumonia	14.16 ± 0.28	13.0 ± 0.5	0 ± 0	18.64
4	P. vulgaris	14.00 ± 0.5	12.33 ± 0.28	0 ± 0	28.92
5	S. aureus	15.0 ± 0.0	13.16 ± 0.28	0 ± 0	29.91
6	P. aeruginosa	15.5 ± 0.5	13.5 ± 0.5	0 ± 0	31.82
7	B. cereus NCIM 5557	12.50 ± 0.5	11.16 ± 0.28	0 ± 0	25.45
8	B. megaterium NCIM 5334	15.33 ± 0.28	13.5 ± 0.5	0 ± 0	28.94
9	M. luteus NCIM 5262	15.5 ± 1.0	14.0 ± 0.5	0 ± 0	22.57
10	B. subtilis NCIM 2920	16.0 ± 1.0	14.0 ± 0.5	0 ± 0	30.61
11	C. albicans	12.0 ± 0.0	11.16 ± 0.28	0 ± 0	15.62
12	A. niger NCIM 1358	13.16 ± 0.28	11.0 ± 0.5	0 ± 0	43.12
13	M. racemosus NCIM 1334	13.0 ± 0.5	11.33 ± 0.28	0 ± 0	31.65
14	P. chrysogenum NCIM 1333	14.0 ± 1.0	10.0 ± 0.5	0 ± 0	96.00
15	R. stolonifer NCIM 1139	14.0 ± 1.0	12.5 ± 1.0	0 ± 0	25.44

All experiments were performed in triplicate
^aThe mean surface area of the growth inhibition zone (mm²) was calculated for AgNPs and AgNO₃ from the mean diameter. The percent of increases of inhibition zone area for the AgNPs against different microorganism were calculated as (y²−x²)/x² × 100, where x and y are the inhibition zone for 20 μl of AgNO₃ and 20 μl of AgNPs respectively

ISSN: 1476-0711