Table 1 List of oligonucleotide primers for amplification of β-lactamase genes
Serial no. | Targets | Primers pairs | Sequence (5′→3′) | Product size (bp) | Reference |
|---|---|---|---|---|---|
1 | DHA-1 and DHA-2 | DHA F DHA R | TGATGGCACAGCAGGATATTC GCTTTGACTCTTTCGGTATTCG | 997 | [18] |
2 | KPC | KPC F KPC R | 5′-CATTCAAGGGCTTTCTTGCTGC-3′ 5′-ACGACGGCATAGTCATTTGC-3′ | 538 | [20] |
3 | IMI/NMC | IMI/NMC F IMI/NMC R | 5′-CCATTCACCCATCACAAC-3′ 5′-CTACCGCATAATCATTTGC-3′ | 440 | [21] |
4 | SME | SME F SME R | 5′-AACGGCTTCATTTTTGTTTAG-3′ 5′-GCTTCCGCAATAGTTTTATCA-3′ | 831 | [22] |
5 | VIM | VIM F VIMR | 5′-GATGGTGTTTGGTCGCATA-3′ 5′-CGAATGCGCAGCACCAG-3′ | 390 | [23] |
6 | IMP | IMP F IMP R | 5′-TTGACACTCCATTTACDG-3′ 5′-GATYGAGAATTAAGCCACYCT-3′ | 139 | [23] |
7 | NDM | NDM F NDM R | 5′-GGGCAGTCGCTTCCAACGGT-3′ 5′-GTAGTGCTCAGTGTCGGCAT-3′ | 476 | [24] |
8 | DHA-1 | DHA-RT F DHA-RT R | 5′-TGATGGCACAGCAGGATATTC-3′ 5′-TACTTACAGATCCGAGCTCAA-3′ | 144 | This study |