Table 1 Gene targets PCR primer and probe sequences
Target gene/symbol | Forward primer | Reverse primer | Probe | Reference |
|---|---|---|---|---|
Intimin (eae) | cattgatcaggatttttctggtgata | ctcatgcggaaatagccgtta | atagtctcgccagtattcgccaccaatacc | [11] |
Shiga toxin 1(stx1) | gtggcattaatactgaattgtcatca | gcgtaatcccacggactcttc | tctgccggacacatag (MGB) | [12]a |
Shiga toxin 2 (stx2) | gggcagttattttgctgtgga | tgttgccgtattaacgaaccc | ctatcaggcgcgttttgaccatcttcg | [13] |
Bundle-forming pilus (bfp) | gcatcattccgttgttgg | ggaccatgtattatcaaaaacctg | ccgccttctgacaagctgtgttgg | [14] |
EHEC factor for adherence 1/lymphocyte inhibitory factor A (efa1/lifA) | tcacaccagaattattacgtcacaca | atggtagtcaggtatacatccgtatttc | accggcacaatactccagactccagaaga | This study |