Fig. 1

Analysis of expressed VirB12 recombinant protein at different times after induction on SDS-PAGE (12% w/v). Lane 1 protein molecular mass marker (KDa). Lane 2 protein expression in Bl21 (DE3) with pET28a vector 1 h after adding IPTG. Lane 3 protein expression in transformed Bl21 (DE3) with pET28a-VirB12 recombinant vector before adding IPTG. Lane 4 protein expression in transformed Bl21 (DE3) with pET28a-VirB12 recombinant vector 1 h after adding IPTG. Lane 5 protein expression in transformed Bl21 (DE3) with pET28a-VirB12 recombinant vector 2 h after adding IPTG. Lane 6 protein expression in transformed Bl21 (DE3) with pET28a-VirB12 recombinant vector 4 h after adding IPTG