Table 3 Primers used to amplify and sequence the seven housekeeping genes in the E. coli isolates for the MLST analysis
Gene | Primer sequences | Usage |
|---|---|---|
adk | F 5′-ATTCTGCTTGGCGCTCCGGG -3′ R 5′-CCGTCAACTTTCGCGTATTT-3′ | Amp/seq |
fumC | F 5′-TCACAGGTCGCCAGCGCTTC-3′ R 5′-GTACGCAGCGAAAAAGATTC-3′ | Amp/seq |
gyrB | F 5′-TCGGCGACACGGATGACGG-3′ R 5′-ATCAGGCCTTCACGCGCATC-3′ | Amp/seq |
icd | F 5′-ATGGAAAGTAAAGTAGTTGTTCCGGCACA-3′ R 5′-GGACGCAGCAGGATCTGTT-3′ | Amp/seq |
mdh | F 5′-ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG-3′ R 5′-TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT-3′ | Amp/seq |
purA | F 5′-CGCGCTGATGAAAGAGATGA-3′ R 5′-CATACGGTAAGCCACGCAGA-3′ | Amp/seq |
recA | F 5′-CGCATTCGCTTTACCCTGACC-3′ R 5′-TCTCGATCAGCTTCTCTTTT-3′ | Amp/seq |