Molecular characterization of Staphylococcus aureus isolates from various healthcare institutions in Nairobi, Kenya: a cross sectional study

Table 1 Primers used in the updated version of SCCmec multiplex PCR

Primer name	Primer sequence (5 33)	Primer specificity (SCCmec type, region)	Amplicon size (bp)	Conc. (µM)
CIF2 F2	TTCGAGTTGCTGATGAAGAAGG	I, J1 region	495	0.4
CIF2 R2	ATTTACCACAAGGACTACCAGC			0.4
ccrC F2	GTACTCGTTACAATGTTTGG	V, ccr complex	449	0.8
ccrC R2	ATAATGGCTTCATGCTTACC			0.8
RIF5 F10	TTCTTAAGTACACGCTGAATCG	III, J3 region	414	0.4
RIF5 R13	ATGGAGATGAATTACAAGGG			0.4
SCCmec V J1 F	TTCTCCATTCTTGTTCATCC	V, J1 region	377	0.4
SCCmec V J1 R	AGAGACTACTGACTTAAGTGG			0.4
dcs F2	CATCCTATGATAGCTTGGTC	I, II, IV, and VI, J3 region	342	0.8
dcs R1	CTAAATCATAGCCATGACCG			0.8
ccrB2 F2	AGTTTCTCAGAATTCGAACG	II and IV, ccr complex	311	0.8
ccrB2 R2	CCGATATAGAAWGGGTTAGC			0.8
kdp F1	AATCATCTGCCATTGGTGATGC	II, J1 region	284	0.2
kdp R1	CGAATGAAGTGAAAGAAAGTGG			0.2
SCCmec III J1 F	CATTTGTGAAACACAGTACG	III, J1 region	243	0.4
SCCmec III J1 R	GTTATTGAGACTCCTAAAGC			0.4
mecI P2	ATCAAGACTTGCATTCAGGC	II and III, mec complex	209	0.8
mecI P3	GCGGTTTCAATTCACTTGTC			0.8
mecA P4	TCCAGATTACAACTTCACCAGG	Internal positive control	162	0.8
mecA P7	CCACTTCATATCTTGTAACG			0.8

ISSN: 1476-0711