Comparison of methods for the identification of microorganisms isolated from blood cultures

Table 1 Primers used for the identification of some bacterial species by PCR

Microorganism	Gene	Annealing temperature (°C)	Amplicon size (bp)	Reference
Acinetobacter baumannii	^blaOXA_51-like	52	353	[44]
Acinetobacter lwoffii	^blaOXA_154-like	52	223	[45]
Enterobacter cloacae	Hsp60 (housekeeping)	52	343	[46]
Enterococcus faecalis	16S chromosomal region	60	143	[47]
Enterococcus faecium	sodA (superoxide dismutase)	45	216	[21]
Escherichia coli	gadA (alpha-glutamate decarboxylase)	65	373	[48]
Klebsiella pneumoniae	rpoB (β subunit of RNA polymerase)	52	364	[49]
Morganella morganii	16S chromosomal region	62	809	[50]
Proteus mirabilis	rsbA (quorum sensing)	55	236	[51]
Pseudomonas aeruginosa	Genome fragment	55	724	[52]
Serratia marcescens	16S chromosomal region	52	1058	[53]
Staphylococcus aureus	16S chromosomal region	52	442	[54]
Stenotrophomonas maltophilia	atpD (housekeeping)	52	854	[55]
Bacillus spp.	rpoB (β subunit of RNA polymerase)	72	400	[14]
Enterobacter aerogenes	16SrDNA	55	280	[15]

ISSN: 1476-0711