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Table 6 Numbers of detected conjugative TEM genes

From: Genetic characterization of TEM-type ESBL-associated antibacterial resistance in Enterobacteriaceae in a tertiary hospital in Ghana

Isolate

TEM

Number that had transferred by conjugation

Citrobacter spp.

0

0

Enterobacter spp.

1

0

Escherichia coli

11

3

Klebsiella spp.

12

8

Proteus spp.

1

1

  1. The TEM primer produced PCR amplification product in DNA extracts from 25 of the ESBL-positive isolates. Verification of which of these genes had been transferred by conjugation was obtained by comparing this data to results of PCR on plasmid-DNA extracts from the transconjugants
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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