Table 1 Comparison of viral and Bordetella pathogen detection rate among cohorts of patients with different tests ordered by clinicians
From: Comparison of molecular detection methods for pertussis in children during a state-wide outbreak
Cohort 1: pertussis PCR alone N = 584 (% positive) | Cohort 2: viral PCR alone N = 3071 (% positive) | Cohort 3: dual tested N = 490 (% positive) | p value | |
|---|---|---|---|---|
Median age | 3 years | 2 years | 0.54 years | <0.001 |
Age range | 6 days–21 years | 3 days–21 years | 2 days–20 years | NA |
Patient age <24 months | 240 (41 %) | 1268 (41 %) | 369 (75 %) | <0.001 |
Bordetella pathogen detection by LD-PCR (%) | n = 21 (3.6 %), including 3 B. parapertussis a | NA | n = 22 (4.5 %), including 2 B. parapertussis a | 0.5 |
B. pertussis detection by RM-PCR (%) | NA | n = 4 (0.1 %) | n = 24 (4.9 %) | <0.001 |
Combined detection by both RM-PCR and LD-PCR: n (%) | NA | NA | n = 27 (5.5 %), including 2 B. parapertussis a | NA |
Organism isolation by culture: n (% of culture positive over detection by LD-PCR) | n = 14 (14/21 or 66.7 %), including 2 B. parapertussis a | NA | n = 10 (10/22 or 45.5 %), including one B. parapertussis a | 0.16 |
Rate of viral positive detection: n (%) | NA | 1988 (64.6 %) | 367 (74.8 %) | <0.001 |
Rate of viral positive in patients age < 24 months: n/n (%) | NA | 840/1268 (66.2 %) | 290/369 (78.6 %) | <0.001 |
Flu A | NA | 164 (5.3) | 17 (3.5) | <0.001 (combined Flu A and B rates) |
Flu B | NA | 360 (11.7) | 30 (6.1) | |
PIV 1-4 | NA | 83 (2.7) | 33 (6.7) | <0.001 |
RSV | NA | 433 (14.1) | 124 (25.3) | <0.001 |
HMPV | NA | 186 (6.0) | 35 (7.1) | 0.4 |
AdV | NA | 88 (2.9) | 17 (3.5) | 0.5 |
RhV | NA | 741 (24.1) | 143 (29.2) | 0.02 |
CoV | NA | 111 (3.6) | 24 (4.9) | 0.17 |
2–3 Viruses | NA | 170 (5.5) | 54 (11.0) | <0.001 |