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Table 1 Phenotypic and molecular characterization results of IRMS and MRIS P. aeruginosa isolates

From: Characterization of Pseudomonas aeruginosa with discrepant carbapenem susceptibility profile

Micro No.

Imipenem MIC

(µg/ml)

Meropenem MIC

(µg/ml)

Doripenem MIC

(µg/ml)

CarbaNP test

MHT

Multiple × PCRa

oprD mutations

RT-qPCR oprD

(fold difference)

RT-qPCR

mexAB

(fold difference)

Sequence

Types-ST

(MLST)

Imipenem resistant meropenem susceptible P. aeruginosa (IRMS)

 

 PA3970

4

4

4

Negative

Negative

Negative

L4, L6

2.31

–

–

 PA5778

6

1.5

1

Negative

Negative

Negative

L4, L6

0.81

–

ST 1685

 PA4745

>32

4

2

Negative

Negative

Negative

L2, L7, L8, L9, L15

Undetermined

–

–

 PA6192

4

2

0.5

Negative

Negative

Negative

L2, L7, L8, L9, L15

0.16

–

ST 1993

 PA6362

4

1

1

Negative

Negative

Negative

L4, L6

1.27

–

ST 360

 PA6475

32

2

1.5

Negative

Negative

Negative

Mutations in primer binding site

Undetermined

–

ST 1952

 PA6476

4

1

1

Negative

Negative

Negative

L2, L7, L8,

L9, L15

0.39

–

ST 617

Meropenem resistant imipenem susceptible P. aeruginosa (MRIS)

 

 PA4381

6

4

3

Negative

Negative

Negative

Not done

Not done

3.37

–

 PA5350

4

32

4

Negative

Negative

Negative

Not done

Not done

2.54

–

 PA26815

3

8

12

Negative

Negative

Negative

Not done

Not done

1.09

–

  1. Genes tested: blaIMP,blaVIM, blaNDM, blaKPC, blaOXA-48 like genes. Relative quantification of oprD and mexAB expression was normalized with ATCC 27853 P. aeruginosa with a value of 1.0 and the fold difference was mentioned above