Table 1 Oligonucleotide primers used for genotyping of Helicobacter pylori strains isolated from ready to eat food samples
Genes name | Primer Sequence (5′–3′) | Size of product (bp) |
|---|---|---|
vacA s 1 a | F: CTCTCGCTTTAGTAGGAGC R: CTGCTTGAATGCGCCAAAC | 213 |
vacA s 1 b | F: AGCGCCATACCGCAAGAG CTGCTTGAATGCGCCAAAC | 187 |
vacA s 1 c | F: CTCTCGCTTTAGTGGGGYT R: CTGCTTGAATGCGCCAAAC | 213 |
vacA s 2 | F: GCTAACACGCCAAATGATCC R: CTGCTTGAATGCGCCAAAC | 199 |
vacA m 1 A | F: GGTCAAAATGCGGTCATGG R: CCATTGGTACCTGTAGAAAC | 290 |
vacA m 1 B | F: GGCCCCAATGCAGTCATGGA R: GCTGTTAGTGCCTAAAGAAGCAT | 291 |
vacA m 2 | F: GGAGCCCCAGGAAACATTG R: CATAACTAGCGCCTTGCA | 352 |
cag A | F: GATAACAGCCAAGCTTTTGAGG R: CTGCAAAAGATTGTTTGGCAGA | 300 |
iceA 1 | F: GTGTTTTTAACCAAAGTATC R: CTATAGCCASTYTCTTTGCA | 247 |
iceA 2 | F: GTTGGGTATATCACAATTTAT R: TTRCCCTATTTTCTAGTAGGT | 229/334 |
oip A | F: GTTTTTGATGCATGGGATTT R: GTGCATCTCTTATGGCTTT | 401 |