Clinical features and characteristics of Clostridium difficile PCR-ribotype 176 infection: results from a 1-year university hospital internal ward study

Table 1 Microbiological and molecular characteristics of C. difficile isolates

CDI case no.	Isolate no.	GDH	Toxin A/B	Anaerobic culture	PCR-ribotype	Toxin gene presence (A/B/bin)^a,b	Vancomycin MIC (mg/L)	Metronidazole MIC (mg/L)
1	n/a	+	+	–	n/a	n/a	n/a	n/a
2	259	+	+	+	176	A/B/bin	0.25	0.25
3	263	+	+	+	176	A/B/bin	0.25	0.25
4	269	+	+	+	176	A/B/bin	0.5	0.5
5	205	+	+	–	n/a	n/a	n/a	n/a
6	273	+	+	+	176	A/B/bin	0.6	0.25
7	298	+	–	+	049	A/B	1	0.5
8	280	+	+	–	n/a	n/a	n/a	n/a
9	279	+	+	+	176	A/B/bin	1	0.25
10	294	+	+	+	176	A/B/bin	0.12	1
11	303	+	+	+	176	A/B/bin	0.25	0.5
12	277	+	–	+	001	A/B	0.5	0.5
13	304	+	+	+	014	A/B	0.25	0.5
14	44	+	–	+	002	A/B	0.25	0.5
15	307	+	+	+	017	A/B	0.25	0.25
16	308	+	+	+	176	A/B/bin	0.25	0.25
17	316	+	+	+	176	A/B/bin	0.5	0.25
18	320	+	–	+	012	A/B	0.5	0.5
19	319	+	+	+	012	A/B	0.5	0.12
20	322	+	+	+	176	A/B/bin	0.5	2
21	365	+	–	+	176	A/B/bin	0.5	2
22	323	+	+	+	020	A/B	0.5	0.5
23	325	+	+	+	015	A/B	0.5	0.25
24	331	+	+	+	078	A/B/bin	0.5	0.25
25	336	+	+	+	176	A/B/bin	0.5	1
26	351	+	+	+	176	A/B/bin	0.25	1
27	365	+	–	+	014	A/B	0.5	0.5
28	391	+	+	+	005	A/B	0.25	0.5
29	388	+	+	+	434	A/B	0.25	0.12
30	248	+	+	+	176	A/B/bin	0.12	0.5

GDH glutamate dehydrogenase, MIC minimum inhibitory concentration
^aToxin A/toxin B/binary toxin
^bPrimers used to amplify tcdA are located upstream of the repetitive region in the 3′-end. The TcdA-negative strains due to 3′-end deletion revealed positive PCR amplification [13]

ISSN: 1476-0711