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Table 2 Primers used in this study for amplification of class 1 and class 2 integrons and other antibiotic resistant genes

From: Molecular characterization of selected multidrug resistant Pseudomonas from water distribution systems in southwestern Nigeria

Primer pair

Target

Sequence (5´–3´)

Annealing temperature (â—¦C)

Amplicon size (bp)

References

Primer 1 16s-8F

16S rDNA

AGAGTTTGATCMTGGCTCAG

60

456

[36]

Primer 2 16s-517

16S rDNA

ATTACCGCGGCTGCTGG

60

456

[37]

IntI1_F

Class 1 integrase gene

CCTCCCGCACGATGATC

55

270

[38]

IntI1_R

TCCACGCATCGTCAGGC

IntI2_F

Class 2 integrase gene

TTATTGCTGGGATTAGGC

50

233

[39]

IntI2_R

ACGGCTACCCTCTGTTATC

5′_CS

Class 1 integron variable region

GGCATCCAAGCAGCAAG

58.5

Variable

[40]

3′_CS

AAGCAGACTTGACCTGA

Hep_F

Class 2 integron variable region

CGGGATCCCGGACGGCATGCACGATTTGTA

60

Variable

[41]

Hep_R

GATGCCATCGCAAGTACGAG

Sul1-F

sul1

CGGCGTGGGCTACCTGAACG

60

433

[42]

Sul1-R

GCCGATCGCGTGAAGTTCCG

Sul2-F

sul2

GCGCTCAAGGCAGATGGCATT

60

293

[42]

Sul2-R

GCGTTTGATACCGGCACCCGT

pVP440sul3F

sul3

TCAAAGCAAAATGATATGAGC

55

787

[42]

pVP440sul3R

TTTCAAGGCATCTGATAAAGAC

qacEΔ1F

qacEΔ1F

ATC GCA ATA GTT GGC GAA GT

58

800

[43]

sul1-B

sul1-B

GCA AGG CGG AAA CCC GCG CC

58

 

[44]

aph (3″)c-F

aph (3″)c

GCTCAAAGGTCGAGGTGTGG

55

515

[27]

aph (3″)c-R

CCAGTTCTCTTCGGCGTTAG

55

515

[27]

ant (3″)b-F

ant (3″)b

CAGCGCAATGACATTCTTGC

55

295

[27]

ant (3″)b-F

GTCGGCAGCGACA(C/T)CCTTCG

55

295

[27]

aph(6)-1dd-F

aph(6)-1dd

GACTCCTGCAATCGTCAAGG

55

560

[27]

aph(6)-1dd-R

GCAATGCGTCTAGGATCGAG

55

560

[27]

tet(A)-F

tet(A)

TTGGCATTCTGCATTCACTC

60

494

[45]

tet(A)-R

GTATAGCTTGCCGGAAGTCG

60

494

[45]

tet(B)-F

tet(B)

CAGTGCTGTTGTTGTCATTAA

60

571

[45]

tet(B)-R

GCTTGGAATACTGAGTGTAA

60

571

[45]

tet(E)-F

tet(E)

TATTAACGGGCTGGCATTTC

55

544

[45]

tet(E)-R

AGCTGTCAGGTGGGTCAAAC

55

544

[45]

tet(M)-F

 

ACACGCCAGGACATATGGAT

55

536

[45]

tet(M)-R

tet(M)

ATTTCCGCAAAGTTCAGACG

55

536

[45]

tet(30)-F

tet(30)

CCGTCATGCAATTTGTGTTC

55

550

[45]

tet(30)-R

TAGAGCACCCAGATCGTTCC

55

550

[45]

SHV_F

bla SHV

GCGAAAGCCAGCTGTCGGGC

62

538

[46]

SHV_R

GATTGGCGGCGCTGTTATCGC

62

538

[46]

CTX_F

bla CTX-M

GTGCAGTACCAGTAAAGTTATGG

55

538

[46]

CTX_R

CGCAATATCATTGGTGGTGCC

 

538

[46]

TEM_F

bla TEM

AAAGATGCTGAAGATCA

44

425

[46]

TEM_R

TTTGGTATGGCTTCATTC

 

425

[46]