Table 2 Primers used in this study for amplification of tetracycline-resistance genes (Call et al., 2003)
Primer pair | Target | Sequence (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
tet(A)- F | tet(A) | TTGGCATTCTGCATTCACTC | 60 | 494 | Call et al., 2003 |
tet(A)- R | Â | GTATAGCTTGCCGGAAGTCG | 60 | 494 | ,, |
tet(B)- F | tet(B) | CAGTGCTGTTGTTGTCATTAA | 60 | 571 | ,, |
tet(B)- R | Â | GCTTGGAATACTGAGTGTAA | 60 | 571 | Â |
tet(E)- F | tet(E) | TATTAACGGGCTGGCATTTC | 55 | 544 | ,, |
tet(E)- R | Â | AGCTGTCAGGTGGGTCAAAC | 55 | 544 | ,, |
tet(M)- F | tet(M) | ACACGCCAGGACATATGGAT | 55 | 536 | ,, |
tet(M)- R | Â | ATTTCCGCAAAGTTCAGACG | 55 | 536 | ,, |
tet(30)- F | tet(30) | CCGTCATGCAATTTGTGTTC | 55 | 550 | ,, |
tet(30)- R | Â | TAGAGCACCCAGATCGTTCC | 55 | 550 | ,, |
Tet(O)-F | tet(O) | GCGGTAATTATGGGAAACGA | 55 | 550 | ,, |
Tet(O)-R | Â | TTTCCCGCTGTTCAGATTTC | Â | Â | Â |