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Table 1 CLSI methodology for broth microdilution

From: Use of vancomycin as a surrogate for dalbavancin in vitro susceptibility testing: results from the DISCOVER studies

Testing conditions

Dalbavancin

Vancomycin

Medium

Cation-adjusted Mueller Hinton Broth supplemented with 0.002% (v/v) polysorbate 80

Cation-adjusted Mueller Hinton Broth

Inoculum

Direct colony suspension, equivalent to a 0.5 McFarland standard

Direct colony suspension, equivalent to a 0.5 McFarland standard

Incubation

35 +/− 2°C; ambient air; 24 hours

35 +/− 2°C; ambient air; 24 hours

Solvent/diluent

DMSO

Water