Table 1 Primer sequence used for screening of genes coding ESBL or carbapenemase
SHPT@ESBL-1(SHV, TEM, CTX-M) | Final concentration (mM) | ||
|---|---|---|---|
590 | SHPT108@CTX-M-U1-F | ATGTGCAGYACCAGTAARGTKATGGC | 0.4 |
SHPT108@CTX-M-U2-R | GGTRAARTARGTSACCAGAAYCAGCGG | 0.4 | |
422 | SHPT108@TEM-U-F | TCGCCGCATACACTATTCTCAGAATGAC | 0.08 |
SHPT108@TEM-U-R | CAGCAATAAACCAGCCAGCCGGAAG | 0.08 | |
739 | SHPT108@TR-SHV-F | TGTATTATCTC(C/T)CTGTTAGCC(A/G)CCCTG | 0.48 |
SHPT108@TR-SHV-R | GCTCTGCTTTGTTATTCGGGCCAAGC | 0.48 | |
SHPT@ESBL-2(VEB, GES, PER) | |||
391 | SHPT108@TR-VIM-F | GATGGTGTTTGGTCGCATATCGCAAC | 0.08 |
SHPT108@TR-VIM-R | CATCGCTGTTGGGGTTGCCCAATTTT | 0.08 | |
604 | SHPT108@TR-SPM-F | CTGGCAGGGATCGCTCACTC | 0.08 |
SHPT108@TR-SPM-R | GGTTTCCGATCAGCCACCTCTCA | 0.08 | |
731 | SHPT108@NDM-1-F primer | CAGTGTGGGGGCCTGACGAT | 0.08 |
SHPT108@NDM-1-R primer | CTGAGCA ACC TGC GCA ATR ATA GCT T | 0.08 | |
SHPT@Carba-1(NDM, SPM, VIM) | |||
320 | SHPT108@TR-VIM-F | GATGGTGTTTGGTCGCATATCGCAAC | 0.04 |
SHPT108@TR-VIM-R | CGAATGCGCAGCACCAGGATAGAA | 0.04 | |
291 | SHPT108@TR-SPM-F | CGTTTGAAAATCTGGGTACGCAAACG | 0.04 |
SHPT108@TR-SPM-R | GTTTCAAATCAAAAACATTATCCGCTGGAACAG | 0.04 | |
200 | SHPT108@NDM-1-F primer | CGAAAGTCAGGCTGTGTTGCGC | 0.08 |
SHPT108@NDM-1-R primer | GACCGCCCAGATCCTCAACTG | 0.08 | |
SHPT@Carba-2(IMP, AIM, KPC/BIC, DIM) | |||
710 | SHPT108@Tr-DIM-F | TATTCAGCTTGTCTTCGCTTGCTAACG | 0.08 |
SHPT108@Tr-DIM-R | GTTAGCGTTCGGCTGGATTGATTTG | 0.08 | |
412 | TR-KPC-BIC-F | GCTTTCT(T/G)GCTG(C/G)CGC(T/C)GTGCT | 0.2 |
TR-KPC-BIC-R | AGCCAATCAAC(A/C)A(A/G)CTGCTG(C/A)CGC | 0.2 | |
326 | SHPT108@AIM-F | CCCTGAAGGTGTACGGAAACAC | 0.04 |
SHPT108@AIM-R | GGGTTCGGCCACCTCGAATTG | 0.04 | |
204 | Tr-IMP-F | AC(G/A)GG(C/G/T)GGAATAGAGTGGCTTAA(T/C)TCTC | 0.02 |
TR-IMP-R | TTCAGG(C/T)A(A/G)CCAAACYACTASGTTATCT | 0.02 | |
SHPT@Carba-3(Oxa23 like group, Oxa48 like group, Oxa58 like group) | |||
599 | SHPT108@Oxa-58-F | CCCCTCTGCGCTCTACATACAACATC | 0.08 |
SHPT108@Oxa-58-R | AAGTATTGGGGCTTGTGCTGAGCATAG | 0.08 | |
482 | Tr-OXA-G23-F2 | AGAATATGT(G/C)CC(A/T)GC(C/A)TC(T/A)ACATTTAA(A/G)ATG | 0.2 |
TR-OXA-G23-R2 | CCCA(G/A)CC(G/T)GT(C/T)AACCA(G/A)CC | 0.2 | |
286b | Tr-Oxa-G48-F2 | CACCAAGTCTTTAAGTGGGATGGACA | 0.08 |
Tr-Oxa-G48-R2 | CCGATACGTGTAACTTATTGTGATACAGCTT | 0.08 | |