Table 1 The sequence of primers used in this study
Target gene | Primer | Sequence | product size(bp) |
|---|---|---|---|
blaOXA-51-like | OXA-51-like F | TAATGCTTTGATCGGCCTTG | 353 |
OXA-51-like R | TGGATTGCACTTCATCTTGG | ||
blaOXA-23-like | OXA-23-like F | GATCGGATTGGAGAACCAGA | 501 |
OXA-23-like R | ATTTCTGACCGCATTTCCAT | ||
blaOXA-24-like | OXA-24-like F | TTCCCCTAACATGAATTTGT | 1024 |
OXA-24-like R | GTACTAATCAAAGTTGTGAA | ||
blaOXA-58-like | OXA-58-like F | TGGCACGCATTTAGACCG | 507 |
OXA-58-like R | AAACCCACATACCAACCC | ||
blaIMP-1 | IMP F | CTACCGCAGCAGAGTCTTTAC | 587 |
IMP R | AACCAGTTTTGCCTTACCAT | ||
blaVIM-1 | VIM F | ATTCCGGTCGGMGAGGTCCG | 633 |
VIM R | GAGCAAGTCTAGACCGCCCG | ||
blaSHV | SHV F | GGTTATGCGTTATATTCGCC | 865 |
SHV R | TTAGCGTTGCCAGTGCTC | ||
blaGES | GES F | ATGCGCTTCATTCACGCAC | 392 |
GES R | ATTTGCTGATTTCGCTCGG | ||
blaTEM | TEM F | ATCAGCAATAAACCAGC | 516 |
TEM R | CCCCGAAGAACGTTTTC | ||
16S rRNA | 16S-8F | AGAGTTTGATCCTGGCTCAG | 1499 |
16S-1493R | ACGGCTACCTTGTTACGACTT | ||
blaAmpC | AmpC F | CGACAGCAGGTGGAT | 513 |
AmpC R | GGTTAAGGTTGGGATG | ||
aac(6′)-Ib | aac(6′)-Ib F | ATGACTGAGCATGACCTTGC | 519 |
aac(6′)-Ib R | TTAGGCATCACTGCGTGTTC | ||
aac(6′)-II | aac(6′)-II F | GAGCGACCGACTCTTGATG | 326 |
aac(6′)-II R | CGTATGGCTCGATGGTTGTT | ||
CarO | CarO F | CAGAGCCTTTTCCTAAGGAGAA | 916 |
CarO R | GCTCACCTGATGCTGACATTAA | ||
qacΔE 1-sul1 | qacEΔ1-sul1 F | TAGCGAGGGCTTTACCTAAGC | 300 |
qacEΔ1-sul1 R | ATTCAGAATGCCGAACACCG | ||
intl 1 | intl 1 F | ACGAGCGCAAGGTTTGGT | 565 |
intl 1 R | GAAAGGTCTGGTCATACATG |