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Table 1 Primers and PCR conditions used to amplify resistance genes

From: Antimicrobial susceptibility and resistance mechanisms of methicillin resistant Staphylococcus aureusisolated from 12 Hospitals in Turkey

Resistance genes

Primers

PCR conditions

References

ermA

F 5′TCT AAA AAG CAT GTA AAA GAA3′

Pre cycle 93°C 3 min,

[7] Sutcliffe 1996

R 5′CTT CGA TAG TTT ATT AAT ATT AGT3′

35′cycles: 93°C 60 s, 52°C 60 s, 72°C 60 s

ermB

F 5′GAA AAG GTA CTC AAC CAA ATA3′

Last cycle 72°C 5 min

R 5′AGT AAC GGT ACT TAA ATT GTT TAC3′

ermC

F 5′GCT AAT ATT GTT TAA ATC GTC AAT TCC3′

R 5′GGA TCA GGA AAA GGA CAT TTT AC3′

msrA

F 5′GCA AAT GGT GTA GGT AAG ACA ACT3′

Pre cycle 93°C 3 min,

[7] Sutcliffe 1996

R 5′ATC ATG TGA TGT AAA CAA AAT3′

35′cycles: 93°C 30 s, 52°C 30 s, 72°C 60 s

Last cycles 72°C 10 min

linA

F 5′GTA TTA ACT GGA AAA CAG CAA AG3′

Pre cycle 5 dk 94°C

[10] Lina 1999

R 5′GAG CTT CTT TTG AAA TAC ATG G3′

35′cycles 45′s 94°C, 45′s 54°C,1 min at 72°C

linB

F 5′CCTACCTATTGTTTGTGGAA 3′

Last cycle 5 min at 72°C

[11] Bozdogan 1999

R 5′ATAACGTTACTCTCCTATTC 3′

tetM

F 5′GTG GAC AAA GGT ACA ACG AG3′

Pre cycle 93oC’de 5 dk

[9] Warsa 1996

R 5′CGG TAA AGT TCG TCA CAC AC3′

35′cycles 93°C 60 s, 52°C 60 s, 72°C 60 s

tetK

F 5′CAG CAG ATC CTA CTC CTT3′

Last cycle 10 min at 72°C

R 5′TCG ATA GGA ACA GCA GTA3′

aac-aph

F 5′GAG CAA TAA GGG CAT ACC AAA AAT C3′

Pre cycle 94C’de 5 dk,

[8] Kao 2000

R 5′CCG TGC ATT TGT CTT AAA AAA CTG G3′

35′cycles 94°C 30 s, 50°C 30 s, 72°C 30 s

Last cycle 7 min at 72°C