Resistance genes | Primers | PCR conditions | References |
---|---|---|---|
ermA | F 5′TCT AAA AAG CAT GTA AAA GAA3′ | Pre cycle 93°C 3 min, | [7] Sutcliffe 1996 |
R 5′CTT CGA TAG TTT ATT AAT ATT AGT3′ | 35′cycles: 93°C 60 s, 52°C 60 s, 72°C 60 s | ||
ermB | F 5′GAA AAG GTA CTC AAC CAA ATA3′ | Last cycle 72°C 5 min | |
R 5′AGT AAC GGT ACT TAA ATT GTT TAC3′ | |||
ermC | F 5′GCT AAT ATT GTT TAA ATC GTC AAT TCC3′ | ||
R 5′GGA TCA GGA AAA GGA CAT TTT AC3′ | |||
msrA | F 5′GCA AAT GGT GTA GGT AAG ACA ACT3′ | Pre cycle 93°C 3 min, | [7] Sutcliffe 1996 |
R 5′ATC ATG TGA TGT AAA CAA AAT3′ | 35′cycles: 93°C 30 s, 52°C 30 s, 72°C 60 s | ||
Last cycles 72°C 10 min | |||
linA | F 5′GTA TTA ACT GGA AAA CAG CAA AG3′ | Pre cycle 5 dk 94°C | [10] Lina 1999 |
R 5′GAG CTT CTT TTG AAA TAC ATG G3′ | 35′cycles 45′s 94°C, 45′s 54°C,1 min at 72°C | ||
linB | F 5′CCTACCTATTGTTTGTGGAA 3′ | Last cycle 5 min at 72°C | [11] Bozdogan 1999 |
R 5′ATAACGTTACTCTCCTATTC 3′ | |||
tetM | F 5′GTG GAC AAA GGT ACA ACG AG3′ | Pre cycle 93oC’de 5 dk | [9] Warsa 1996 |
R 5′CGG TAA AGT TCG TCA CAC AC3′ | 35′cycles 93°C 60 s, 52°C 60 s, 72°C 60 s | ||
tetK | F 5′CAG CAG ATC CTA CTC CTT3′ | Last cycle 10 min at 72°C | |
R 5′TCG ATA GGA ACA GCA GTA3′ | |||
aac-aph | F 5′GAG CAA TAA GGG CAT ACC AAA AAT C3′ | Pre cycle 94C’de 5 dk, | [8] Kao 2000 |
R 5′CCG TGC ATT TGT CTT AAA AAA CTG G3′ | 35′cycles 94°C 30 s, 50°C 30 s, 72°C 30 s | ||
Last cycle 7 min at 72°C |