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Table 3 Sensitivity and specificity tested using multiplex real-time PCR and conventional PCR in 11860 clinical samples

From: Rapid genetic typing of diarrheagenic Escherichia coli using a two-tube modified molecular beacon based multiplex real-time PCR assay and its clinical application

DEC group

Multiplex real-time PCR

Standard PCR

Total

Sensitivity

95% CI

Specificity

95% CI

Kappa

+1

2

ETEC

+

29 (a)

9 (b)

38

100%

88.06 ~ 100.00

99.92%

99.86 ~ 99.97

0.87

-

0 (c)

11822 (d)

11822

  

Total

29

11831

11860

  

EPEC

+

99

12

111

100%

96.34 ~ 100.00

99.90%

99.82 ~ 99.95

0.94

-

0

11749

11749

  

Total

99

11761

11860

  

EHEC

+

4

0

4

100%

39.76 ~ 100.00

100%

99.97 ~ 100.00

1.00

-

0

11856

11856

  

Total

4

11856

11860

  

EAEC

+

10

0

10

100%

69.15 ~ 100.00

100%

99.97 ~ 100.00

1.00

-

0

11850

11850

  

Total

10

11850

11860

  

EIEC Positive

+

8

0

8

100%

63.06 ~ 100.00

100%

99.97 ~ 100.00

1.00

-

0

11852

11852

  

Total 171

8 150

11852

11860

  
  1. +1: Positive, −2: Negative.