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Table 2 The reproducibility of the two-tube multiplex real-time PCR assay

From: Rapid genetic typing of diarrheagenic Escherichia coli using a two-tube modified molecular beacon based multiplex real-time PCR assay and its clinical application

genes

106 cfu/ml

105 cfu/ml

104 cfu/ml

103 cfu/ml

Mean Ct & SD

CV (%)

Mean Ct & SD

CV (%)

Mean Ct & SD

CV (%)

Mean Ct & SD

CV (%)

eaeA

21.22 ± 0.18

0.85

24.58 ± 0.22

0.90

28.14 ± 0.31

1.10

31.26 ± 0.26

0.83

escV

20.8 ± 0.13

0.63

24.49 ± 0.11

0.45

27.73 ± 0.25

0.90

30.99 ± 0.32

1.03

ipaH

22.11 ± 0.18

0.81

25.85 ± 0.31

1.12

29.93 ± 0.22

0.74

34.34 ± 0.458

1.31

Stx1/2

19.64 ± 0.18

0.92

23.16 ± 0.09

0.39

26.44 ± 0.21

0.79

29.74 ± 0.2

0.67

aggR

22.02 ± 0.17

0.77

25.52 ± 0.31

1.21

28.95 ± 0.39

1.35

32.3 ± 0.28

0.87

lt

22.06 ± 0.14

0.63

25.29 ± 0.31

1.23

28.99 ± 0.22

0.76

32.13 ± 0.49

1.53

sth

22.04 ± 0.0.13

0.58

25.11 ± 0.31

1.23

28.54 ± 0.4

1.40

32.07 ± 0..25

0.78

stp

25.2 ± 0.09

0.36

28.76 ± 0.29

1.01

32.19 ± 0.23

0.71

35.1 ± 0.21

0.60