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Table 1 Detection limits and amplification efficiency of multiplex rtPCR assay

From: Rapid genetic typing of diarrheagenic Escherichia coli using a two-tube modified molecular beacon based multiplex real-time PCR assay and its clinical application

Tube

Pathotypes

Virulence gene

Equation

R2

Efficiency

Liner range (CFU/mL)

Detection limits (CFU/mL)

Tube 1

ETEC

stp

y = −3.345 × +45.797

0.9964

97.3%

3.0 × 102 ~ 3.0 × 106

3.0 × 102

  

sth

y = −3.386 × +42.136

0.9993

97.4%

3.0 × 102 ~ 3.0 × 106

3.0 × 102

  

lt

y = −3.343 × +42.537

0.9992

99.1%

3.0 × 102 ~ 3.0 × 107

3.0 × 102

 

EAEC

aggR

y = −3.371 × +42.398

0.9997

99.7%

2.0 × 102 ~ 2.0 × 106

2.0 × 102

Tube 2

EHEC

stx1/2

y = −3.257 × +39.610

0.9994

102.8%

8.8 × 101 ~ 8.8 × 106

8.8 × 101

EPEC

eaeA

y = −3.405 × +41.464

0.9996

96.6%

8.8 × 102 ~ 8.8 × 106

8.8 × 102

escV

y = −3.475 × +41.401

0.9991

94.0%

8.8 × 102 ~ 8.8 × 106

8.8 × 102

EIEC

ipaH

y = −3.523 × +45.832

0.9973

92.2%

6.8 × 102 ~ 6.8 × 106

6.8 × 102