Effects of antibacterial mineral leachates on the cellular ultrastructure, morphology, and membrane integrity of Escherichia coli and methicillin-resistant Staphylococcus aureus

Table 2 Summary of MRSA survival and membrane permeability following incubation in TSB, water, pH 3.6 phosphate buffer, and mineral leachates

Treatment	Time (h)	Flow cytometry, percent intact membranes (avg ± SD)^a	CFU enumeration (% viability reduction)^b
TSB	15	96.6 (95.8 ± 2.6)	2-log₁₀ Increase (n/a)^c
dH₂O	15	61.9 (78.0 ± 16.0)	1-log₁₀ Decrease (90.0)
pH 3.6 Buffer	15	6.1 (2.6 ± 3.0)	5-log₁₀ Decrease (99.999)
CB07-L	15	3.2 (3.3 ± 2.4)	4-log₁₀ Decrease (99.99)
BY07-L	15	1.7 (1.7 ± 1.0)	4.5-log₁₀ Decrease (99.995)

^a Actual percentages based on 50,000 total cells analyzed by flow cytometry following 15 h exposure to experimental condition in triplicate experiments.
^b Actual values presented as log₁₀ comparison of viability between 0 h and 15 h exposure to experimental conditions (Figure 6b). Percent viability values were calculated based on the change in viability between 0 h and 15 h exposure for each experimental condition (Figure 6b).
^c Percentage of viable cells was not applicable due to cell growth in TSB over time.

ISSN: 1476-0711