Effects of antibacterial mineral leachates on the cellular ultrastructure, morphology, and membrane integrity of Escherichia coli and methicillin-resistant Staphylococcus aureus

Table 1 Summary of E. coli survival and membrane permeability following incubation in LB, water, pH 3.6 phosphate buffer, and mineral leachates

Treatment	Time (h)	Flow cytometry, percent intact membranes (avg ± SD)^a	Plate count viability, CFU enumeration (%)^b
LB	1	99.9 (99.9 ± 0.1)	0.5-log₁₀ Increase (n/a)^c
dH₂O	1	99.5 (99.0 ± 0.6)	< 0.5-log₁₀ Decrease (50.0)
pH 3.6 Buffer	1	95.4 (92.3 ± 5.4)	< 0.5-log₁₀ Decrease (50.0)
CB07-L	1	96.4 (93.0 ± 6.6)	1.5-log₁₀ Decrease (5.0)
BY07-L	6	99.3 (99.9 ± 0.3)	2.5-log₁₀ Decrease (0.5)

^a Actual percentages based on 50,000 total cells analyzed by flow cytometry following exposure to experimental conditions in triplicate experiments.
^b Actual values presented as log₁₀ comparison of viability between 0 h and 1 or 6 h exposure to experimental conditions (Figure 6a). Percent viability values were calculated based on the change in viability between 0 h and 1 or 6 h exposure for each experimental condition (Figure 6a).
^c Percentage of viable cells was not applicable due to cell growth in LB over time.

ISSN: 1476-0711